Lysosomal permeabilization by Group A <i>Streptococcus</i> releases proteins into the macrophage cytosol.

ABSTRACT: The human-specific bacterial pathogen Group A Streptococcus (GAS) is a significant cause of morbidity and mortality due to its ability to cause severe invasive infection. Although macrophages are important for controlling GAS infection, we and others have demonstrated that GAS can persist in macrophages by perforating the phagolysosome using the pore-forming toxin streptolysin O (SLO). In this study, we examined how phagosomal perforation releases lysosomal and bacterial proteins into the cytosol and alters cytosolic protein content in the macrophage. Using IL-1β as a measure of intracellular pathogen detection, we confirmed that cytosolic preparations from macrophages infected with either wild-type (WT) or SLO-deficient (ΔSLO) bacteria contained new proteins that are absent in unin

SUBMITTER: Quezada A 

PROVIDER: S-EPMC12633395 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature