Engineering HIV antibodies with enhanced breadth and potency of neutralization through multistate affinity maturation.

ABSTRACT: Broadly neutralizing antibodies (bnAbs) against HIV hold promise as therapeutic and prophylactic agents, but realizing this potential requires antibodies that function across the antigenic heterogeneity of the HIV envelope glycoprotein (Env). Although numerous bnAbs have been isolated from infected individuals, their breadth and potency may not be sufficient to tackle global viral diversity, motivating efforts to further improve their neutralization capacity. Here, we address this challenge using a multistate antibody engineering approach integrating deep mutational scanning with combinatorial library screening across diverse Env variants. This strategy enables identification of mutation patterns that confer improved binding across antigenically distinct targets. Starting from one of the b

SUBMITTER: Kedzior M 

PROVIDER: S-EPMC12633424 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature