MRNA delivery of genetically encoded mosaic-8 pan-sarbecovirus RBD vaccines.

ABSTRACT: Global health remains threatened by spillovers of zoonotic SARS-like betacoronaviruses (sarbecoviruses) that could be mitigated by a pan-sarbecovirus vaccine¹. We described elicitation of potently neutralizing and cross-reactive anti-sarbecovirus antibodies by mosaic-8 nanoparticles (NPs) displaying eight different sarbecovirus spike receptor-binding domains (RBDs) as 60 copies of eight individual RBDs^2-6 (mosaic-8 RBD-NPs) or 30 copies of two "quartets," each presenting four tandemly-arranged RBDs⁷ (dual quartet RBD-NPs). To facilitate manufacture of a broadly protective mosaic-8 vaccine, we generated membrane-bound RBD quartets that can be genetically encoded and delivered via mRNA: dual quartet RBD-mRNA and dual quartet RBD-EABR-mRNA, which utilizes ESCR

SUBMITTER: Cohen AA 

PROVIDER: S-EPMC12633426 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature