Phosphoproteomic Profiling Reveals Overlapping and Distinct Signaling Pathways in <i>Dictyostelium discoideum</i> in Response to two different chemorepellents.

ABSTRACT: Chemorepulsion causes cells to move away from the source of a signal, but the underlying mechanisms for eukaryotic cells are poorly understood. We performed proteomics and phosphoproteomics to elucidate how Dictyostelium discoideum responds to its two endogenous chemorepellent signals, the protein AprA and inorganic polyphosphate (polyP). At 60 minutes, AprA increased levels of 211 proteins and reduced levels of 57 proteins while polyP increased levels of 152 proteins and reduced levels of 168 proteins. Surprisingly, many of the AprA- and polyP-regulated proteins are associated with RNA metabolism and ribosomes. AprA and polyP both upregulated 19 proteins, one protein was downregulated by both, and one was upregulated by AprA and downregulated by polyP. AprA increased phosphorylatio

SUBMITTER: Uddin SZ 

PROVIDER: S-EPMC12633543 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature