Argonautes and small RNAs associated with nematode programmed DNA elimination.

ABSTRACT: Programmed DNA elimination (PDE) selectively removes portions of the genome during development, deviating from the genome constancy rule. Our understanding of PDE mechanisms, including the involvement of small RNAs and transposases, is mainly based on studies in the single-cell ciliates, whereas in metazoans, the PDE mechanisms remain largely unknown. In the parasitic nematode Ascaris, PDE removes and remodels all germline chromosome ends, splits fused chromosomes into somatic chromosomes, leading to dramatic karyotype changes. To probe the possible involvement of small RNAs in Ascaris PDE, we identified two Argonautes, AsWAGO-2 and AsWAGO-3, associated with PDE. Using cellular fractionation followed by nuclease-treated immunoprecipitation, we characterized WAGO-associated sm

SUBMITTER: Zagoskin MV 

PROVIDER: S-EPMC12633612 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature