High-fidelity microsecond-scale cellular imaging using two-axis compressed streak imaging fluorescence microscopy.

ABSTRACT: Compressed streak imaging (CSI) is a computational imaging strategy that can acquire video at over 150 trillion frames per second. Despite this achievement, CSI faces challenges in detecting subtle intensity fluctuations in slow-moving, continuously illuminated objects. This limitation, largely attributable to high streak compression and motion blur, has curtailed the broader adoption of CSI in cellular fluorescence microscopy. To address these issues and expand the utility of CSI, we developed a two-axis compressed streak imaging (TACSI) method that results in significant improvements to the reconstructed video fidelity. TACSI introduces a second scanning axis which shuttles a conjugate image of the object with respect to the coded aperture. The moving image decreases the streak compressi

SUBMITTER: Keppler MA 

PROVIDER: S-EPMC12633632 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature