Characterization of Early Metastatic Subpopulations in Uveal Melanoma: Single-Cell Insights Into SPP1+ Cells and Their Interactions With Macrophages.

ABSTRACT:

Purpose

To identify highly invasive subpopulations of tumor cells that may be responsible for early uveal melanoma (UM) metastasis at the single-cell level and to analyze their interactions with immune cells.

Methods

Single-cell RNA sequencing was conducted on 11 samples from 11 UM patients who underwent primary enucleation. The patients were categorized into metastasis (M+) and nonmetastasis (M-) groups based on whether metastasis occurred during the 3-year follow-up. The results were validated using bulk RNA sequencing data from 133 patients from our cohort (n = 53) and The Cancer Genome Atlas human UM database (n = 80). The clustering was confirmed by immunofluorescence. Cell proliferation and invasiveness were evaluated using CCK8 and transwell assays. Apoptosis was anal

SUBMITTER: Luo J 

PROVIDER: S-EPMC12633778 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature