5-hydroxymethylcytosines from circulating cell-free DNA as noninvasive prognostic markers for gastric cancer.

ABSTRACT:

Background

Prognostic assessment plays a crucial role in guiding clinical management and treatment decisions for gastric cancer patients. The enrichment characteristics of 5-hydroxymethylcytosine (5hmC) in circulating cell-free DNA (cfDNA) has emerged as potential prognostic epigenetic markers.

Methods

Using 5hmC-Seal combined with next-generation sequencing (NGS), we profiled the genome-wide distribution of 5hmC in plasma cfDNA samples from 51 gastric cancer patients. Prognostic biomarkers were selected via random survival forest and Cox proportion hazards models, and a prognostic model was subsequently constructed.

Results

Seven prognostic biomarker genes were identified, and the 7-gene prognostic model demonstrated a concordance index (C-index) of 0.892 (95% CI = 0

SUBMITTER: Fu Y 

PROVIDER: S-EPMC12633862 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature