Characterization of alveolar epithelial cells type II during postnatal lung development in relation to alveolarization - Stereological studies of rat lungs.

ABSTRACT:

Objective

Rats are born with morphologically immature lungs, but intact surfactant system. The aim of this study was to characterize the surfactant producing alveolar epithelial cells type II (AEII) during alveolarization and find relationships between the intracellular surfactant pool and alveolar surface area, lung volume and body weight.

Methods

After exsanguination, lungs of 3, 7, 14, 21 and 90 days old rats were inflated with a pressure of 10 mm H20 and fixed by perfusion and prepared for light and electron microscopy. Using different stereological parameters AEII were characterized.

Results

At day 21, the end of bulk alveolarization, the alveolar surface and the number of AEII increased significantly but their volume and size did not change compared to values be

SUBMITTER: Huttmann J 

PROVIDER: S-EPMC12633894 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature