Multispecific antibodies: Bioanalytics for early-stage screening and characterization of mispairing profiles.

ABSTRACT: Multispecific antibodies (MsAbs) enable the targeting of different epitopes, representing a strategy with enhanced therapeutic potential. However, the intracellular assembly of MsAbs is complex and generates unwanted mispaired species, imposing a significant burden on downstream processing and analytical characterization, thus increasing the overall timeline and cost of bioprocess development. The establishment of analytical tools to aid in the characterization and understanding of MsAb mispairing profiles at early-stage screenings is mandatory. Here, we implemented mass spectrometry (MS) and high-performance liquid chromatography (HPLC) methods to assess mispairing levels using several Chinese Hamster Ovary (CHO) clones producing a MsAb. Results showed that both methods are suitable to be

SUBMITTER: Melo C 

PROVIDER: S-EPMC12633938 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature