<i>Puribacter membranae</i> gen. nov., sp. nov., isolated from a biofilm of a membrane bioreactor (MBR) treating sewage.

ABSTRACT: Novel Gram-stain-negative, non-spore-forming, non-motile rods, designated HTMS2 and HTMS3^T, were isolated from a biofilm on the membrane of a municipal sewage treatment membrane bioreactor in Nagaoka, Japan. Phylogenetic analysis of 16S rRNA genes placed them in the family Burkholderiaceae, most closely related to Hydromonas duriensis A2P5 ^T (94.14% similarity). Genome sequencing (2.52 Mb, 48.2% G+C) and phylogenomic analysis affiliated them with the uncultured genus lineage CTSOIL-112 in the Genome Taxonomy Database. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values to related taxa were below species thresholds (ANI ≤77.0%, dDDH ≤54.9%). In addition, average amino acid identity values to related taxa were ≤68.6%. Both str

SUBMITTER: Hatamoto M 

PROVIDER: S-EPMC12634028 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature