Frozen section histopathology and preanalytical factors affecting nucleic acid integrity in biobanked fresh-frozen human cancer tissues.

ABSTRACT:

Background

In this study, we evaluated the effects of storage duration and ischemic time on nucleic acid quality of fresh-frozen tissue (FFT) from colon adenocarcinoma (COAD), hepatocellular carcinoma (HCC), and renal cell carcinoma (RCC) collected at the Cancer Tissue Bank of Seoul National University Hospital.

Methods

A total of 102 FFT samples were analyzed to compare DNA integrity number (DIN) and RNA integrity number (RIN) according to storage duration and ischemic time. Additionally, the effects of histopathologic features-such as tumor cell proportion, inflammatory cell infiltration, and stromal fibrosis-on nucleic acid quality were evaluated.

Results

DIN and RIN remained stable overall even though the storage duration increased, with no statistically significa

SUBMITTER: Kim S 

PROVIDER: S-EPMC12634257 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature