Polysaccharides from <i>Rosa roxburghii</i> Tratt fruit: The effect of extraction solvent on their chemical structure, bioactivities, <i>in vitro</i> simulated digestion and fecal fermentation characteristics.

ABSTRACT: Polysaccharides extracted from Rosa roxburghii Tratt fruit (RTFPs) using hot water, 0.9 % NaCl, 5 % NaOH/0.05 % NaBH₄ or citric acid (pH 3.0) were systematically compared for their structure, antioxidant and hypoglycemic capacities, simulated gastrointestinal stability, and human-fecal fermentation behavior. Citric-acid extraction yielded a low-molecular-weight, uronic-acid-rich fraction that exhibited the strongest DPPH, ABTS, hydroxyl and superoxide radical-scavenging activities and the lowest IC₅₀ values against α-glucosidase and α-amylase. During in-vitro digestion RTFPs remained largely intact, yet fermentation of the citric fraction selectively enriched Bacteroides, Phascolarctobacterium and Dialister, enhanced acetate, propion

SUBMITTER: Chen G 

PROVIDER: S-EPMC12634302 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature