Evaluation of an antibody panel for alpha-synuclein detection in FFPE rectal biopsies in Parkinson's disease.

ABSTRACT:

Background

Conflicting results have been published regarding the accumulation of alpha-synuclein (aSyn) pathology, in intestinal tissues of patients with Parkinson's disease (PD). This study investigates the use of a panel of aSyn antibodies for detecting pathological aSyn in rectal biopsy samples from PD patients and healthy individuals.

Materials and methods

A panel of antibodies targeting native, phosphorylated and conformation-specific forms of aSyn was used to characterize aSyn distribution in the substantia nigra and colonic tissues of controls and individuals with Lewy pathology. Distribution of aSyn was further analyzed in formalin-fixed and paraffin-embedded (FFPE) submucosal rectal biopsies of 24 PD patients and 20 healthy controls.

Results

All tested antibo

SUBMITTER: Schroder K 

PROVIDER: S-EPMC12634310 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature