Microbial degradation of citrate mediates sealing of cement cracks under anaerobic conditions relevant to radioactive waste disposal.

ABSTRACT: In radioactive waste repositories, cement is used for construction, backfill, and waste encapsulation. Over time, cracks may form, creating potential pathways for contaminant migration. A self-sealing mechanism is through calcium carbonate (CaCO₃) precipitation, which can be driven by microbial oxidation of organic compounds. We explored microbially induced calcite precipitation facilitated by metabolism of citrate, a complexant in low- and intermediate- level radioactive waste (L/ILW). Nitrate-reducing microcosms containing cement pellets, citrate, nitrate, alkaline sediment inoculum, and synthetic groundwater (pH 11.2) were incubated in the dark (20 °C, 40 days). Aqueous geochemical data revealed complete citrate removal, denitrification, pH decrease to pH 9, and removal of Ca

SUBMITTER: Byrd N 

PROVIDER: S-EPMC12634444 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature