Optimized delivery of RNA silencing prolyl hydroxylase domain 2 for enhanced angiogenesis and osteogenesis using bioactive glass scaffolds.

ABSTRACT: This study investigated the delivery of small interfering RNA (siRNA) silencing prolyl hydroxylase domain 2 (PHD2) via poly(lactic-co-glycolic acid) (PLGA)-bioactive glass scaffold to improve angiogenesis and osteogenesis for biomineralized tissue engineering. Polyethylenimine (PEI) was complexed with PHD2siRNA at 3:1, 6:1, and 8:1 ratios, and the resulting complexes were characterized by transmission electron microscopy, NanoSight analysis, and gel retardation assays. Their biological effects on human umbilical vein endothelial cells (HUVECs) were evaluated to determine the optimal ratio. PLGA-bioactive glass scaffolds were prepared and loaded with the most effective PEI/PHD2siRNA complex. HUVECs were used to confirm controlled RNA release from the scaffolds. The angiogenic effects of HUV

SUBMITTER: Kunwong N 

PROVIDER: S-EPMC12634668 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature