Characterization of the bone marrow architecture of multiple myeloma using spatial transcriptomics.

ABSTRACT: The bone marrow (BM) is a complex and compartmentalized tissue where spatial context plays a critical role in regulating cell behavior, signaling, and disease progression. To capture these dynamics, we apply spatial transcriptomics using the Visium Spatial Gene Expression platform on formalin-fixed paraffin-embedded (FFPE) BM sections from both healthy and Multiple Myeloma (MM) mouse models, as well as MM patient samples. Overcoming the technical challenges of working with mineralized long bone tissue, we develop a custom analytical framework integrating spatial and single-cell transcriptomic data to map cellular composition and interactions in situ. This approach enables the spatial characterization of transcriptionally heterogeneous malignant plasma cells (MM-PC) and their surrounding mi

SUBMITTER: Muinos-Lopez E 

PROVIDER: S-EPMC12634688 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature