Fluorescence-Based Online Monitoring Enables Differentiation in Co-Cultures of Untagged Streptomyces Species and Trichoderma reesei.

ABSTRACT: Microbial co-cultures provide significant advantages over commonly used axenic cultures in biotechnological processes, including increased productivity and access to novel natural products. However, differentiated quantification of the microorganisms in co-cultures remains challenging using conventional measurement techniques. To address this, a fluorescence-based approach was developed to enable the differentiated online monitoring of microbial growth in co-cultures. Co-cultures of Streptomyces species (Streptomyces sp./spp.) with Trichoderma reesei (T. reesei) were investigated. T. reesei was tagged with the fluorescent protein mCherry, while Streptomyces spp. were used as untagged wild-type strains. First, two-dimensional (2D) fluorescence spectra of the individual microorganisms were r

SUBMITTER: Brauneck G 

PROVIDER: S-EPMC12634738 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature