R-Spondin1 Regulates Fate of Enteric Neural Progenitors via Differential LGR4/5/6 Expression in Mice and Humans.

ABSTRACT:

Background & aims

Regeneration and cytodifferentiation of various adult epithelial stem cell compartments are controlled by the WNT agonist R-Spondin1 (RSPO1) and the Leucin-rich-repeat-containing G-protein-coupled receptors (LGR4/5/6). We hypothesized that RSPO1-LGR signaling is also involved in regulating neuroregeneration and homeostasis of the postnatal enteric nervous system (ENS).

Methods

We isolated neural crest-derived ENS cells from wnt1-tomato mice and patient samples, which were evaluated using pharmacological in vitro studies under RSPO1 stimulation. We use proliferation assays (BrdU incorporation, Ki67 staining), as well as neuronal differentiation screenings. We performed fluorescence-activated cell sorting-based in vitro assays to stratify human ENS cells for

SUBMITTER: Scharr M 

PROVIDER: S-EPMC12634853 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature