Chick embryo extract-driven culture system enhances chicken muscle satellite cell differentiation and 3D myotube formation.

ABSTRACT: The rapid increase in global meat demand has placed significant pressure on traditional meat production. This has led to the emergence of the cultured meat industry as a promising alternative offering sustainable and ethical solutions. This study explored the efficacy of chick embryo extract (CEE) as a potential economic supplement. Specifically, we aimed to identify the optimal culture conditions for chicken muscle satellite cells (CMSCs) using CEE and evaluate their effects on cell proliferation and differentiation. The CEE+horse serum (HS) condition was found to be the most effective for CMSC proliferation, with a significant increase in the expression of myogenic transcription factors. CMSCs cultured in 30% CEE + 3% HS-containing differentiation medium (CEE + 3%HS-DM) showed higher exp

SUBMITTER: Kang Y 

PROVIDER: S-EPMC12635065 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature