Pendrin inhibition is associated with protective effect of prone positioning in a ventilator-induced lung injury mouse model.

ABSTRACT: Pendrin (SLC26A4), a transmembrane anion exchanger, is upregulated in inflammatory airway diseases. In this study, we analyzed the role of pendrin expression in a ventilator-induced acute lung injury (VILI) animal model. VILI was induced in the supine or prone position by a high tidal volume (HTV) of 30 mL/kg for 5 h in pendrin wild-type (WT) and knockout (KO) 129SVEV mice. Pendrin inhibitor (YS-01) was intraperitoneally administered to modulate pendrin signaling. Lung injury parameters were assessed based on bronchoalveolar lavage fluid (BALF) analysis, inflammatory cytokine analysis by ELISA, and histopathological findings. Pendrin expression was determined by western blotting and transmission electron microscopy (TEM) using immunogold labeling methods. The degree of lung injury was sign

SUBMITTER: Choi JS 

PROVIDER: S-EPMC12635137 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature