A comprehensive evaluation of metabolically engineered Cynara cardunculus calli as platform for valuable fatty acid derivatives production.

ABSTRACT: Increasing global demand for energy along with climate change claims for sustainable and renewable energy sources. In this study, we investigated transcriptomic changes in two cardoon (Cynara cardunculus L. var. altilis) calli cell culture lines engineered for production of valuable fatty acids, therefore suitable for large-scale production of biofuel precursors, as alternative to triacylglycerols (TAGs) engineered plants. Namely, to gain insight in the molecular effects of genetic transformation, we analyzed the transcriptome of SAD-OE line (overexpressing stearic acid desaturase), and FAD-KD line (a fatty acid desaturase RNA interference line, FAD2.2-RNAi) accumulating oleic and linoleic acids, respectively. Differentially expressed genes (DEGs) involved in fatty acid metabolism and stre

SUBMITTER: Pirona R 

PROVIDER: S-EPMC12635154 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature