Polarized ATP synthase in synaptic mitochondria induced by learning and plasticity signals.

ABSTRACT: MINFLUX is a cutting-edge single-molecule localization microscopy technology that surpasses the conventional diffraction limit, enabling nanostructure visualization with exceptional precision. However, its application has largely been limited to cultured cells. In this research, we refined sample preparation protocols for 3D MINFLUX imaging in fixed brain tissue, focusing on mitochondrial distribution within dendritic spines and engram cells of the dentate gyrus. Probing single molecules reveals that mitochondrial inner membrane proteins reorganize during synaptic plasticity in dendritic spines of engram cells. Using 3D MINFLUX nanoscopy, we identified a significant redistribution of α-F1-ATP synthase, correlating with learning-related activities. This redistribution suggests a pivotal rol

SUBMITTER: Hu Y 

PROVIDER: S-EPMC12635157 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature