Novel antimicrobial peptides and peptide-microbiome crosstalk in Appalachian salamander skin.

ABSTRACT: Using multi-omics tools, we discovered new antimicrobial peptides (AMPs) and examined AMP-microbial interactions in three Appalachian salamander species (Plethodon cinereus, Eurycea bislineata and Notophthalmus viridescens). We conducted skin transcriptomics (n = 13) and proteomics (n = 91) to identify 200+ candidate AMPs. With candidate AMPs, we identified correlations with skin microbiomes and synthesized 20 peptides to challenge against pathogens of amphibians (Batrachochytrium dendrobatidis: Bd) and humans (ESKAPEE). Using transcriptomics, candidate AMPs were detected in all individuals with Cathelidicins being most common. Using proteomics, AMPs were found in 34% of individuals (31/91)-predominately E. bislineata-with Kinin-like peptides being most common. Candidate AMP composition ge

SUBMITTER: Muletz-Wolz CR 

PROVIDER: S-EPMC12635268 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature