The actin nucleation promoting factor WASH facilitates clathrin-independent endocytosis of human papillomaviruses.

ABSTRACT: Endocytosis is a fundamental cellular process facilitated by diverse mechanisms. Remarkably, several distinct clathrin-independent endocytic processes have been identified and characterized following virus uptake into cells. For some, however, mechanistic execution and biological function remain largely unclear. This includes an endocytic process exploited by human papillomavirus type 16 (HPV16). Using HPV16, we examine how vesicles are formed by combining systematic cellular perturbations with electron and video microscopy. Cargo uptake occurs by uncoated, inward-budding pits. Mechanistically, vesicle scission is facilitated by actin polymerization controlled through the actin nucleation-promoting factor WASH. While WASH typically functions in conjunction with the retromer complex on endo

SUBMITTER: Brinkert P 

PROVIDER: S-EPMC12635285 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature