Nitric oxide modulates UDP-evoked cytosolic transients via P2Y₆ purinoceptor stimulation.

ABSTRACT: Glycosaminoglycans (GAGs) and proteoglycans are key components of the extracellular matrix, playing structural and regulatory roles in various biological processes. GAGs are involved in modulating endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) production. CHO-745 cells, deficient in GAGs, produce significantly higher NO levels than wild-type CHO-K1 cells. Since NO is crucial for vascular function, this study investigated how NO overproduction in GAG-deficient cells affects cytosolic Ca²⁺ transients triggered by P2Y6 receptor (P2Y6R) activation with UDP, a vascular tone regulator. Results showed that GAG-deficient CHO-745 cells experience basal oxidative and nitrosative stress. Although integrin expression was similar, the RGD peptide increased NO production in CH

SUBMITTER: Correa T 

PROVIDER: S-EPMC12635301 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature