Peptide-Based Fluorescent Biosensing System for the Detection of the Melanoma Biomarker S100B.

ABSTRACT: Cutaneous melanoma, responsible for 80% of skin cancer mortality, presents urgent diagnostic challenges due to insufficient early detection methods. Current clinical methods rely on invasive biopsies, while noninvasive approaches primarily serve as adjunctive decision-support tools rather than definitive diagnostics. Here, a peptide-based fluorescent biosensing system was developed for the sensitive and rapid detection of S100B, a key prognostic biomarker for melanoma. Our system employs a fluorescently labeled peptide beacon designed for Förster resonance energy transfer (FRET)-based detection, achieving a subnanomolar detection limit (∼0.045 nM) and great selectivity in human serum samples. Peptide synthesis was performed using optimized solid-phase protocols, enabling precise sequence a

SUBMITTER: Chatzilakou E 

PROVIDER: S-EPMC12635975 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature