CAF derived IL-33 mediated EMT to promote the metastasis of LSCC cells.

ABSTRACT:

Background

The metastasis of laryngeal squamous cell carcinoma (LSCC) is not only caused by the tumor itself but is also closely related to cancer-associated fibroblasts (CAFs). The epithelial-mesenchymal transition (EMT) serves as a key event during its metastasis. However, the specific mechanisms underlying LSCC metastasis remain uncertain.

Methods

A wound healing assay was utilized to evaluate the migratory capacity of LSCC cells (TU686 and TU212 cells). Immunofluorescence staining and Western blot analysis were conducted to demonstrate the expression levels of associated proteins. Migration and invasion assays were employed to assess the migration and invasion abilities of LSCC cells in vitro. A nude mouse metastasis model was used to detect LSCC metastasis in vivo.

R

SUBMITTER: Yang L 

PROVIDER: S-EPMC12636156 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature