Toward simple, rapid, and deep plant proteome analysis with an in-cell proteomics strategy.

ABSTRACT: Although modern mass spectrometry (MS) based proteomics has been broadly employed by the plant science community, proteomics analysis of plant tissues remains highly challenging to general plant biology laboratories, mainly due to the lack of effective and easily adaptable strategies that can process plant samples for MS analysis rapidly and reliably. Existing methods often involves lengthy mechanical disruption and protein extraction, which not only require large starting materials but also can cause significant variations, compromising quantitation accuracy. Here, we present an "in-cell proteomics" strategy and benchmark its performance against conventional processing methods using several model plants, including Arabidopsis thaliana, Nicotiana benthamiana, Zea mays

SUBMITTER: Adekanye D 

PROVIDER: S-EPMC12636300 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature