Longitudinal, simultaneous wide-field fluorescent Ca2+ imaging and fMRI in awake mice.

ABSTRACT: Functional magnetic resonance imaging (fMRI) can be applied in mice and humans making it a key technology in translational neuroimaging research. Yet, most neuroimaging studies in rodents use anesthesia to limit subject motion and stress. This puts a hard boundary on the range of brain and behavioral states that can be studied in animals, and deviates from the near-universal practice of imaging people whilst awake. Recent years have seen a push towards the development of acclimation protocols for imaging mice without anesthesia, but the results have been mixed. In parallel, imaging mice without anesthesia has become routine for complementary neuroimaging methods including wide-field fluorescence calcium (WF-Ca²⁺) imaging. We present the first longitudinal protocol for simultaneo

SUBMITTER: Mandino F 

PROVIDER: S-EPMC12636302 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature