Cerenkov luminescence microscopy: A novel approach for high-resolution radiotracer imaging.

ABSTRACT: This study introduces a novel imaging system utilizing Cerenkov luminescence microscopy (CLM) to visualize the activity of radioactive tracers in 3D tumor spheroids. The system integrates an EMCCD camera, a microscope objective, and a precise sample positioning setup, enabling the high-resolution detection of Cerenkov light emission from radiotracers in a light-tight environment. The characteristics of the new CLM system have been tested on glioblastoma multiforme (GLM) spheroids, subjected or not to X-ray radiotherapy. The system effectively differentiated irradiated from non-irradiated spheroids by detecting distinct variations in signal intensity, which were further validated through comparison with autoradiography and quantitative activity measurements. This demonstrates the system's a

SUBMITTER: Alborghetti L 

PROVIDER: S-EPMC12636394 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature