Control of Synaptic Communication through Molecularly Engineered Bioluminescence Light Emission and Sensing.

ABSTRACT: Synapses are sites of intercellular communication between neurons and from neurons to target organs, and of signal integration that underly physiological and behavioral responses. We have developed a modular platform, Interluminescence (Int), for experimental control of synaptic transmission: bioluminescent light, generated by a luciferase oxidizing a luciferin, from a presynaptic neuron is used to activate transsynaptic optogenetic ion channels in the postsynaptic neuron. Two strategies can activate or silence postsynaptic neurons in vivo in the presence of luciferin. In the 'Act-Int' approach, a luciferase is genetically expressed in synaptic vesicles and released during depolarization-induced presynaptic vesicle fusion and exocytosis. In the 'Persist-Int' approach, a luciferase i

SUBMITTER: Slaviero AN 

PROVIDER: S-EPMC12636396 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature