Suspended Tissue Engineering with Assemblable Microfluidics (STEAM).

ABSTRACT: Suspended tissue culture systems enable cellular responses to mechanical forces critical for tissue development and function. Tissues develop in a complex environment containing both mechanical and chemical cues that vary spatiotemporally; modeling both of these physiochemistries in vitro through integration of spatial patterning with mechanical manipulation is thus an important aspect in microphysiological tissue modeling. Here we introduce Suspended Tissue Engineering with Assemblable Microfluidics (STEAM), a modular tissue fabrication platform that allows for spatially heterogeneous suspended tissue architectures. With STEAM we achieve tissue constructs with multiple regions through the addition of capillary pinning features to control hydrogel precursor flow. STEAM tissues can e

SUBMITTER: Haack AJ 

PROVIDER: S-EPMC12636406 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature