Two-Step Mechanism of Bruton's Tyrosine Kinase Membrane Recruitment and Activation.

ABSTRACT: Peripheral membrane proteins (PMPs) are critical mediators of signaling cascades initiated via activation of cell-surface receptors. Their functions rely on their innate ability to interact with membranes dynamically in response to rapidly changing cellular conditions. This membrane recruitment may occur via high-affinity interactions with specific lipids or transient, low-affinity membrane interactions. These weak and dynamic interactions that are critical regulators of protein function are challenging to capture. Taking Bruton's Tyrosine Kinase (BTK), a non-receptor tyrosine kinase essential for B cell activation, as an example, we demonstrate a native mass spectrometry (nMS) platform to understand the recruitment of PMPs by directly studying it from lipid bilayers customized to target m

SUBMITTER: McAllister RA 

PROVIDER: S-EPMC12636452 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature