Modelling DNA replication fork stability and collapse using chromatin fiber analysis and the R-ODD-BLOBS program.

ABSTRACT: We describe the anatomy of replication forks by comparing the lengths of synthesized BrdU-labelled DNA in wild type, mrc1Δ and cds1Δ Schizoasaccharomyces pombe. We correlated Rad51 and Cdc45 proteins relative to their positions on the fork, replicated tract, or unreplicated regions. We did this by using chromatin fiber images. These fibers track pixel intensity data, which is analyzed using our program: R-ODD-BLOBS. We compared the lengths of BrdU tracts and proteins, as well as the percentage of Rad51 and Cdc45 colocalization, and compared our results with literature findings. We measured average BrdU lengths consistent with current literature; cds1Δ was the longest at ~2.9 kb (8.6 pixels, px), wild type was ~ 2.5 kb (7.5 px), and mrc1Δ was the shortest at ~1.7

SUBMITTER: Cheng K 

PROVIDER: S-EPMC12636476 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature