Comprehensive Single Molecule View of Transcriptional Dynamics in Development.

ABSTRACT: Dynamic patterns of gene expression drive animal development. These transcriptional dynamics depend on the interplay of diverse developmental enhancers within complex regulatory landscapes. Here, we employ a single-molecule genomic method, Fiber-Seq, to capture the simultaneous transcriptional and regulatory states of millions of 20-30 kb chromatin fibers in the 2-4hr Drosophila embryo. These snapshots of genes and their surrounding regulatory context reveal that the basic unit of transcription at highly transcribed genes is a convoy of 2-14 tightly spaced polymerases (Pol II). Further, a subset of templates exhibits hyperbursting, whereby most or all nucleosomes are evicted from the gene body facilitating maximum rates of transcription. We demonstrate that hyperbursting is achieved

SUBMITTER: Tullius TW 

PROVIDER: S-EPMC12636477 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature