Dual-Channel Event Microscopy for Ultrafast Biological Imaging.

ABSTRACT: Many fundamental biological processes-spanning immune-tumor interactions, neuronal signaling, and microvascular flow-exhibit fast, multiscale dynamics among diverse cell types within three-dimensional tissue environments. Capturing such activity requires imaging systems that simultaneously achieve high temporal resolution, multicolor capability, and volumetric coverage over large fields of view (FOVs). However, existing modalities remain limited by tradeoffs among imaging speed, spectral capacity, depth of field (DOF), and spatial resolution. Here, we present Dual-Channel Event Microscopy (DEM), which integrates digital micromirror device (DMD)-based pulsed illumination, extended-DOF optics, and event-based sensing for ultrafast, dual-channel volumetric imaging across a 2.3 × 1.3 mm^2<

SUBMITTER: Guo R 

PROVIDER: S-EPMC12636556 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature