Dielectrophoresis Reveals Stimulus-Induced Remodeling of Insulin Granule Subpopulations.

ABSTRACT: The pancreatic β-cell contains several functional subpopulations of insulin secretory granules (ISGs). These subpopulations vary in maturity, age, and secretory capacity. Differences in protein and lipid composition of ISGs are correlated with disease but require further study to understand how ISG remodeling regulates normal biology. Due to limitations in traditional separation methods, the extent of these subpopulations, any overlap between them, and how they are affected by insulinotropic signals have not been determined. In this work, we adapted direct current insulator-based dielectrophoresis (DC-iDEP) to separate ISGs isolated from INS-1E cells, an immortalized rat insulinoma cell line model, according to their electrokinetic mobility ratio (EKMr). We were able to separate ISG subpop

SUBMITTER: Archambeau A 

PROVIDER: S-EPMC12636567 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature