Novel antibodies for identification, selection, and manipulation of T cells expressing Whitlow linker-containing CARs.

ABSTRACT:

Background

The translational study of chimeric antigen receptor (CAR) T-cell function, persistence, immunophenotype, and spatial localization after infusion is crucial for understanding factors that influence clinical outcomes. However, research has been limited by a lack of optimized tools to reliably detect CAR-engineered cells. To address this, we developed a novel platform to generate monoclonal antibodies (mAbs) targeting a linker peptide incorporated in single-chain variable fragments (scFvs) of most CAR constructs.

Methods

Using recombinant proteins and scFv linker peptides as immunogens, we generated murine mAbs against the Whitlow linker peptide, capable of binding cells expressing Whitlow linker-containing CARs in both fresh and formalin-fixed paraffin-embedded (FF

SUBMITTER: Kimble EL 

PROVIDER: S-EPMC12636878 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature