Engineered Tandem Thymosin Peptide Promotes Corneal Wound Healing.

ABSTRACT:

Purpose

Thymosin beta-4 (TB4) is a small peptide upregulated in injured tissues, playing roles in cell migration, angiogenesis, inflammation, and oxidative stress. Studies show TB4 significantly promotes corneal wound healing after injury, leading to a clinical trial (RGN-259), with full US Food and Drug Administration approval still pending. Current limitations to TB4-based therapies are a short half-life and high peptide synthesis costs, limiting large-scale applications. Here, we engineered a tandem thymosin beta-4 (tTB4) peptide with improved therapeutic potential and scalability for corneal wound repair.

Methods

tTB4 was produced by fusing two TB4 monomers into a single polypeptide, creating dual G-actin binding domains. Binding activity was investigated using structura

SUBMITTER: Nguyen J 

PROVIDER: S-EPMC12636994 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature