Associations of gut microbiome with endogenous estrogen levels in healthy postmenopausal women.

ABSTRACT:

Purpose

The gut microbiome is a potentially important contributor to endogenous estrogen levels after menopause. In healthy postmenopausal women, we examined associations of fecal microbiome composition with levels of urinary estrogens, their metabolites, and relevant metabolic pathway ratios implicated in breast cancer risk.

Methods

Eligible postmenopausal women (n = 164) had a body mass index (BMI) ≤ 35 kg/m² and no history of hormone use (previous 6 months) or cancer/metabolic disorders. Estrogens were quantified in spot urine samples with liquid chromatography-high resolution mass spectrometry (corrected for creatinine). Bacterial DNA was isolated from fecal samples and the V1-V2 hypervariable regions of 16S rRNA were sequenced on the Illumina MiSeq platform.

SUBMITTER: Yaghjyan L 

PROVIDER: S-EPMC12637211 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature