Multiple Buster transposons from hAT superfamily displaying transposition activities in mammal cells.

ABSTRACT: TcBuster, a member of the Buster family within the hAT superfamily, has been engineered into an effective gene transfer tool for gene therapy applications. To diversify the toolkit available for efficient gene delivery, we conducted a bioinformatics analysis, which indicated that some Buster (BT) transposons exhibit signs of recent activity in Actinopterygii and Arthropoda species. Cell-based transposition assays demonstrated that endogenous transposases from Armadillidium nasatum (AnBT), Hyles vespertilio (HvBT), and Lota lota (LlBT) showed 47 %, 44 %, and 39 % transposition activity, respectively, in comparison to that of piggyBac transposons. Conversely, transposases from Carcinoscorpius rotundicauda (CrBT</

SUBMITTER: Guan Z 

PROVIDER: S-EPMC12637253 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature