Stable electron-irradiated [1-¹³C]alanine radicals for metabolic imaging with dynamic nuclear polarization.

ABSTRACT: Dissolution dynamic nuclear polarization (dDNP) increases the sensitivity of magnetic resonance experiments by >10⁴-fold, permitting isotopically labeled molecules to be transiently visible in magnetic resonance imaging scans. dDNP mechanistically takes place at ~1 K and requires unpaired electrons and microwaves. These electrons are usually chemical radicals, requiring removal by filtration prior to injection into humans. Alternative sources, such as ultraviolet irradiation, generate lower polarization and require cryogenic transport. We present ultrahigh-dose rate electron irradiation as an alternative for generating nonpersistent radicals in alanine/glycerol mixtures. These are stable for months at room temperature, quench spontaneously upon dissolution, are present in dose-d

SUBMITTER: Rooney CHE 

PROVIDER: S-EPMC12637281 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature