Integrating genomics and targeted metabolite profiling to elucidate disease-suppression mechanisms of <i>Bacillus velezensis</i> GFB08.

ABSTRACT: Welsh onion cultivation in Taiwan faces a significant threat from a foliar disease complex caused by three major pathogens: Stemphylium vesicarium, Colletotrichum spaethianum, and C. circinans. As an alternative to conventional fungicides, the biocontrol agent Bacillus velezensis GFB08 was systematically characterised through an integrated genomic and targeted metabolite profiling approach. The 3.89 Mbp genome harbored biosynthetic gene clusters for key lipopeptides, and comparative genomics revealed a mersacidin-like BGC, a feature variably present in B. velezensis, indicating enhanced antimicrobial potential. Metabolite profiling identified fengycin and bacillomycin D as the primary antifungal compounds. Bioassays demonstrated that purified fengycin exhibited potent,

SUBMITTER: Cho YT 

PROVIDER: S-EPMC12637390 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature