Dysregulation of U12-Type Splicing in Lupus Neutrophils.

ABSTRACT:

Objective

Neutrophil dysfunction is a hallmark of systemic lupus erythematosus (SLE), but its molecular basis remains unclear. This study explores transcriptional and post-transcriptional changes in low-density granulocytes (LDGs), a proinflammatory neutrophil subset expanded in SLE, focusing on NADPH oxidase (Nox) function and minor intron splicing.

Methods

LDGs and normal-density neutrophils (NDGs) were isolated from SLE patients and healthy controls (HCs). CYBA (P22phox) expression was evaluated at transcript and protein levels. Nox activity was measured using luminol assays. Bulk RNA sequencing and rMATS software were used to assess alternative splicing, particularly of U12-type intron-containing genes.

Results

CYBA expression was reduced in SLE LDGs (n=11) compar

SUBMITTER: Blanco LP 

PROVIDER: S-EPMC12637451 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature