RNA polymerase II-TFIIE-TFIIH interface functions in transcription start site selection in <i>Saccharomyces cerevisiae</i>.

ABSTRACT: During transcription initiation in Saccharomyces cerevisiae, RNA polymerase II (Pol II) and general transcription factors (GTFs) assemble upstream of transcription start sites (TSSs) to form the pre-initiation complex (PIC). In this model organism, yeast, the PIC selects TSSs through a unidirectional scanning mechanism referred to as promoter scanning. Previous studies have shown that the TFIIH subunit Tfb3 connects TFIIH to the rest of the PIC through interactions with Pol II and the GTF TFIIE. Activities within the PIC that influence TSS selection can do so by control of initiation efficiency at individual TSSs or by control of TSS scanning (either rate of scanning or scanning processivity). To understand how this critical interface withing the PIC participates in scanning, we use

SUBMITTER: Basnet P 

PROVIDER: S-EPMC12637456 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature