On the Mechanism of Ezrin Activation.

ABSTRACT: Ezrin is a peripheral membrane protein that contributes to the organization and stability of cellular membrane structures by reversibly linking the plasma membrane to actin filaments. The formation of this membrane-actin linkage has been experimentally shown to require ezrin N-terminal (FERM) domain binding to PI(4,5)P₂ phospholipid-enriched membrane sites and the phosphorylation of the ezrin C-terminal domain (CTD) at residue T567. Collectively, membrane association and T567 phosphorylation are believed to promote separation of the FERM and CTD domains; however, the underlying molecular mechanism remains less clear. In this study, we investigate the mechanistic steps of ezrin activation and the thermodynamic free energy landscape of FERM-CTD dissociation using enhanced sampling

SUBMITTER: Vasiliauskas D 

PROVIDER: S-EPMC12637478 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature