MRNA 3' UTRs direct microRNA degradation to participate in imprinted gene networks and regulate growth.

ABSTRACT: MicroRNAs direct downregulation of target mRNAs. Sometimes, however, this regulatory paradigm inverts, and a target RNA triggers the degradation of a microRNA. This target-directed microRNA degradation (TDMD) requires ZSWIM8. Zswim8 ^-/- mice exhibit reduced growth and perinatal lethality, accompanied by stabilization of dozens of microRNAs. Nonetheless, studies of TDMD function in mammals have been limited because only two TDMD-triggering RNAs have been identified in mice. Here, we computationally identify and validate five new TDMD-triggering sites in mouse models. One site in Atp6v1g1 and two in Lpar4 direct degradation of miR-335-3p, which shows that in mammals, two sites in the same transcript, and multiple sites in different transcripts, can collaborate

SUBMITTER: Lin DH 

PROVIDER: S-EPMC12637508 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature