Identification Of Protein Cargo in Extracellular Vesicles from Macrophages in Progressing and Regressing Tumors.

ABSTRACT: Exosomes are extracellular vesicles (EVs) that carry bioactive molecules from a cell of origin and may alter gene expression of the acceptor cell via binding to cell surface receptors and/or delivering their cargo into the cell. Tumor-associated macrophages (TAMs) can increase or decrease tumor growth through various mechanisms, but the impact of TAM-EVs on tumors has been difficult to elucidate due to challenges in isolating and culturing TAMs for EV purification. In this study, we set out to uncover the protein identities within EVs from TAMs in progressing and regressing tumors, thereby uncovering EV proteins' roles in TAM-tumor crosstalk. TAMs were purified from tumors using magnetic bead isolation and cultured for up to 9 days. TAMs from regressing tumors were found to be more M1-like

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC12637521 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature