Impact of N⁶-methyladenosine (m⁶A) machinery on HIV-1 replication in primary CD4+ T cells.

ABSTRACT: N⁶-methyladenosine (m⁶A) is the most prevalent internal modification of cellular and viral RNA and is critical to the regulation of its localization, stability, and translation. Previous studies on the role of m⁶A during HIV-1 replication have produced conflicting results. Since m⁶A function can vary dramatically by cell type and state, here we aimed to clarify the role of the m⁶A machinery during HIV-1 replication in primary CD4+ T cells. Using CRISPR-Cas9 we targeted 46 cellular genes implicated in m⁶A or 5-methylcytosine (m⁵C) regulation and measured subsequent HIV-1 replication in primary CD4+ T cells. Only knockout of the m⁶A writer complex auxiliary proteins VIRMA and WTAP, and the m⁶A

SUBMITTER: Jackson-Jones KA 

PROVIDER: S-EPMC12637539 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature